Banca de DEFESA: RENATA PAULA COPPINI DE ALMEIDA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RENATA PAULA COPPINI DE ALMEIDA
DATE: 17/10/2024
TIME: 09:00
LOCAL: Plataforma Virtual - Microsoft Teams - https://meet.google.com/ujx-qfkx-eka
TITLE:

Development of polymeric particles loaded with aqueous extract of Psidium guajava L. leaves, with anti-inflammatory action

KEY WORDS:

P. guajava L. leaf extract; oral disorders; medicinal plants; herbal medicines; polymeric particles.

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The use of medicinal plants has directed pharmaceutical research towards the development of new therapeutic options aimed at combating different pathologies. Psidium guajava L., a species belonging tropical countries, P. guajava is native to South America and has been widely used in folk medicine around the world since ancient times. Ethnopharmacological studies show that the species is used to treat a series of diseases such as gastrointestinal disorders, inflammatory processes, and dermatomucosal conditions, as a hypoglycemic agent and analgesic, among others. In Brazil, the species is mainly used to treat acute diarrhea or dysentery. In addition, there are old reports of its use in the treatment of inflammation of the mouth and throat. The aim of this study was to develop, characterize and evaluate polymeric microparticles containing aqueous extract of P. guajava L. leaves in order to assess their anti-inflammatory action against oral diseases, as well as the safety and stability of a pre-formulation for topical use. Different biological activities were evaluated, as well as the characterization of the extract (EAPG) and the developed microparticles. P. guajava leaves were collected, pulverized, equally divided into four batches and the standardized extract was obtained by infusion. All batches were within the limits recommended by Anvisa for total solids content, yield, moisture content, total polyphenol content reproducibility of the extraction process, also demonstrated by the chromatographic profile of TLC and HPLC-DAD. The chromatographic method employed using HPLC was validated and all parameters evaluated presented values within the acceptance criteria. Chitosan particles loaded with extract were developed based on a predictive response surface model, varying the concentration of the chitosan polymer and the sonication time during the addition of the crosslinking agent. The safety of EAPG and the particles were evaluated by performing cytotoxicity tests using the MTT method. Different biological activities were analyzed. The antioxidant activity of EAPG and the microparticles reducing activity and the iron reducing antioxidant power (FRAP) assay methods. The IC50 of the evaluated antioxidant activity ranged from 6.35 to 7.01 µg/mL for the of concentrations equivalent to 60 µM of Fe2+ were found, ranging from 14.42 to 17.83 µg/mL of the tested samples. Regarding anti-inflammatory activity, good results were obtained regarding the expression of IL-6, both of EAPG and of the developed particles. In the antimicrobial activity evaluation assays, EAPG was tested against different Candida species and presented MIC values of 16.24 mg/mL for C. albicans 90028; 12.25 mg/mL for C. albicans 40277; 6.99 mg/mL for C. famata 40135; 6.03 mg/mL for C. glabrata 40134; 8.15 mg/mL for C. guillermondii and; 7.52 mg/mL for C. krusei. No migratory activity of EAPG was obtained using primary fibroblast cell lines in the scratch test. The stability of the particles was evaluated and the best results were obtained in the sample stored in the refrigerator, when compared to that stored at room temperature. Finally, the in vitro skin permeation of EAPG was evaluated. Given all the results found, it can be suggested that the polymeric particles loaded with EAPG developed have potential for the topical treatment of oral conditions.

COMMITTEE MEMBERS:
Externo à Instituição - BENILSON BELOTI BARRETO - MS
Interna - 2295825 - ANA CAROLINA ACEVEDO POPPE
Externa à Instituição - CLAUDIA MASROUAH JAMAL - UFES
Interno - 1880131 - GUILHERME MARTINS GELFUSO
Presidente - 1562111 - PEROLA DE OLIVEIRA MAGALHAES DIAS BATISTA