Banca de QUALIFICAÇÃO: ROSANGELA MARTINES ECHEVERRIA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ROSANGELA MARTINES ECHEVERRIA
DATE: 26/02/2025
TIME: 14:00
LOCAL: Plataforma Microsoft Teams
TITLE:

“SUSTAINABLE USE OF CERRADO BIODIVERSITY: STUDY OF NATIVE SPECIES FOR DIABETES CONTROL”

KEY WORDS:

“diabetes mellitus; biodiversity; α-glucosidase; α-amylase: Cerrado

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

“The Brazilian biodiversity, particularly within the threatened Cerrado biome, presents significant potential for the discovery of new natural products with pharmaceutical applications that can drive sustainable development. As habitat loss intensifies, the urgency to understand and conserve this rich biodiversity increases. Currently, research into the use of forest and non-forest products is being encouraged due to the new trend towards bioeconomics and unique health, which is strengthened by WHO data, which reports that approximately 80 per cent of the world's population uses medicinal plants to meet their primary health needs. Some diseases have seen an increase in cases in recent years, due to biological and social factors, such as diabetes, which by 2030 could affect 643 million people, which requires improved research into appropriate therapeutic solutions. This study focuses on Conservation Units (CU), in the Federal District, characterised as Sustainable Use, where 10 species were selected for their potential anti-antidiabetic properties: Lepidaplao aurea, Psidium laruotteanum, Trema micrantha, Styrax ferrugineus Nees & Mart., Lobelia brasiliensis, Cordiera sessilis, Aegiphila verticillata, Erytroxylum tortuosum, Croton urucurana e Pterodon pubescens. The proposed method consists of collecting the leaves/flowers of the Cerrado botanical species; to extract the chemical compounds in solvents (aqueous, ethanolic and hexanic); carry out the colourimetric enzyme test (α-glucosidase and α-amylase); to make the biomonitoring study with the most prominent species and identify the chemical compounds by means of instrumental chemical analysis. In the screnning, extracts that exceeded 70 % inhibition, in 1 mg/mL of enzyme, were considered active. Among the species evaluated, the ethanolic extracts (EE) of the leaves of P. laruotteanum (EEP) and E. tortuosum (EEt) showed the best enzyme inhibition in α-glucosidase (98.8% and 93.8%, respectively) and α-amylase (75.3% and 92%, respectively); and the best enzyme IC50 evaluated was in α-glucosidase for both species (EEP=5.07 μg/mL and EEt= 21.60 μg/mL). E. tortuosum was the species selected for biomonitoring, which had the most enzymatically active EtF3 fraction, IC50 = 39.50 μg/mL for α-amylase and IC50 = 9.11 μg/mL for α-glucosidase, with inhibition of the latter enzyme being greater than the positive control (deoxynojirimycin), IC50 = 28.83 μg/mL. Moreover, two compounds were also identified in the E. tortuosum species by HPLC and NMR, rutin and ombuin- 3-O- rutinoside, which have not yet been published. These findings, though preliminary, offer promising perspectives for isolating and characterizing the bioactive compounds responsible for anti-diabetes activity. Such research not only supports pharmaceutical discovery but also underscores the vital role of biodiversity conservation in fostering sustainable development strategies.”

COMMITTEE MEMBERS:
Externa à Instituição - CLAUDIA MASROUAH JAMAL - UFES
Presidente - 2328475 - DAMARIS SILVEIRA
Externo à Instituição - JOAO VICTOR DUTRA GOMES - UCB
Externo ao Programa - 1213894 - LUCAS JUNQUEIRA DE FREITAS MOREL - nullInterna - 3231108 - PAULA MONTEIRO DE SOUZA