Banca de DEFESA: LARISSA BARBOSA DE SOUSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LARISSA BARBOSA DE SOUSA
DATE: 27/02/2025
TIME: 14:00
LOCAL: Sala interna da Direção da FS-UnB
TITLE:

“MTA REPAIR HP AND BIO C REPAIR SEALERS: ASSESSMENT OF ANTIMICROBIAL, CYTOTOXIC, MIGRATION AND CYTOKINE EXPRESSION”

KEY WORDS:

“Bioceramics; Repair sealer; Antimicrobial activity; Biocompatibility; Bioactivity."

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

“Endodontic treatment seeks to eliminate microorganisms from the root canals and prevent reinfections, however failures can occur, leading to perforations or failure in endodontic treatment, often requiring surgical interventions. In this context, bioceramic repair sealers stand out as promising alternatives due to their bioactivity and biocompatibility, favoring tissue regeneration and ensuring better results in endodontic repairs and apical sealings. Therefore, this study evaluated the antimicrobial potential of MTA Repair HP (Angelus) and Bio C Repair (Angelus) and its toxicity, proliferative, migratory and immunomodulatory effects on primary cultures of human periodontal ligament cells (hPDLCs). Initially, extracts of repair sealers were prepared in different dilutions (1:1, 1:2 and 1:4). The antimicrobial activity of repair sealers extracts was tested by evaluating its minimum inhibitory concentration for E. faecalis and C. albicans growth. Subsequently, the cytotoxicity of the repair sealers extracts was verified in hPDLCs culture using the MTT assay. Then, the analysis of proliferative effect and migration was evaluated in hPDLCs culture by tripan blue exclusion technique and scratch assay, respectively. Finally, the effect of repair sealers on gene expression was evaluated by qPCR, of pro-inflammatory cytokines’ tumor necrosis factors (TNF-α), interleukin (IL)-1β, and IL6 and the regulatory cytokine IL-10, in different experimental situations. Results demonstrated that Bio C Repair (Angelus) sealer extract (1:1) and its dilution (1:2) showed 46.56% and 9% inhibition of E. faecalis growth, respectively. No tested dilution of either material was toxic to hPDLCs, allowing a minimum viability of 99.65%. In addition, both repair sealers extracts led to greater cell proliferation and migration in their dilutions (1:2 and 1:4) compared to control, after 48h. They also down regulated the expression of pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) and maintaining the expression of the immunomodulatory cytokine IL-10 under inflammatory conditions. Therefore, Bio C Repair (Angelus) showed limited antimicrobial activity, while both sealers demonstrated biocompatibility, and promoted cell proliferation and migration. Additionally, they modulated the inflammatory response, supporting their potential for endodontic repairs and apical sealing.

COMMITTEE MEMBERS:
Externo ao Programa - 1752418 - JACY RIBEIRO DE CARVALHO JUNIOR - nullExterna à Instituição - Poliana Amanda Oliveira Silva - IOA
Externa à Instituição - STELLA MARIS DE FREITAS LIMA - UCB
Presidente - 3342495 - TAIA MARIA BERTO REZENDE