Banca de DEFESA: GUILHERME DE MELO OLIVEIRA GOMES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GUILHERME DE MELO OLIVEIRA GOMES
DATE: 14/08/2025
TIME: 14:00
LOCAL: A definir
TITLE:

Analysis of cell viability and miR-671 expression in an in vitro model of dopaminergic neurodegeneration induced by MPP⁺

KEY WORDS:

Parkinson’s disease. miR-671. Neurodegeneration. SH-SY5Y cells. microRNA

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by the selective loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies, leading to motor, cognitive, and emotional impairments. Studies suggest that microRNAs (miRNAs) are potential modulators of the pathological processes involved in PD, such as oxidative stress, neuroinflammation, and neuronal apoptosis. Among these, miR-671 has emerged for its regulatory effects on inflammatory pathways. This study aimed to investigate the role of miR-671 in an in vitro neurodegeneration model induced by MPP⁺ and rotenone, using SH-SY5Y cells.

Cell viability was assessed by MTT and CCK-8 assays after exposure to different concentrations of MPP⁺ (0.5 to 2.0 mM) and rotenone (0.1 mM) for 24, 48, and 72 hours. The results showed a significant, time- and dose-dependent reduction in cell viability, with a more pronounced effect at higher concentrations and prolonged exposure. The CCK-8 assay proved to be more sensitive and reproducible, with optimal reading between 3 and 5 hours.

miR-671 expression was quantified by RT-qPCR in cells exposed to 1 mM MPP⁺ for 24 hours. A significant increase in miR-671 expression was observed (mean = 1.448) compared to the control group (mean = 0.878; p < 0.05). These findings suggest that miR-671 may be involved in the cellular response to neurotoxic insult, possibly playing a neuroprotective role. The results reinforce the relevance of miRNAs as potential therapeutic targets and promising biomarkers for PD.

COMMITTEE MEMBERS:
Externa à Instituição - CLARA LUNA FREITAS MARINA - UnB
Externo à Instituição - FERNANDO FRANCISCO BORGES RESENDE - UNICEPLAC
Externa à Instituição - MARY ANN ELVINA XAVIER - UnB
Presidente - 1127261 - RICARDO TITZE DE ALMEIDA