Banca de DEFESA: Juliana Amorim dos Santos

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Juliana Amorim dos Santos
DATE: 27/08/2024
TIME: 14:00
LOCAL: Plataforma Virtual Microsoft Teams
TITLE:

Strategies to enhance wound repair: from cell monoculture models to in vitro biomimetic systems

KEY WORDS:

Wound Healing; Monoculture; Co-culture; 3D bioprinter; Biomimetic systems; Skin and Oral mucosa

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

The repair of wounds involves a complex, highly specialized, multi-phase process of tissue response and regeneration. Complications in this process can lead to improper healing, resulting in abnormal scar tissue, infections, and prolonged recovery, which in turn affect patient quality of life and add financial strain to public healthcare systems. This study aims to employ cell culture techniques to explore emerging strategies on wound healing. The research is organized into three chapters, as following: (1) The Chapter 1 aims to evaluate the effects of curcumin in keratinocytes and fibroblasts exposed to ionizing radiation and bacterial stimuli. Scratch assays, cell viability tests, and gene expression analyses were performed using monoculture and co-culture models. The findings showed the importance of cell-cell communication for wound healing. Curcumin enhanced keratinocyte wound closure and cell viability, while presented variable influences on fibroblast response. The gene expression involved in repair pathways showed that curcumin distinctly modulates keratinocytes and fibroblasts. These findings suggest the potentials of curcumin in tissue repair highlighting the need for more research into its therapeutic use during radiotherapy treatments. (2) Chapter 2 aims to summarize existing methods used to bioprint 3D skin equivalents loaded with keratinocytes and fibroblasts. This methodology systematic review included 19 studies published between 2018 and 2023. The studies reported a variety of protocols for skin equivalents using different multilateral bioinks. Gelatin, fibrinogen and alginate were the most used components, possibly due to their mechanical properties with crosslink alternatives. Extrusion bioprinting was the most employed technique and the applied assays varied from bioink characterization, such as rheological prosperities and printability, to animal implantation with cell track and tissue analysis. Although bioprinting of cell-loaded skin equivalents has made significant strides, several challenges remain for its application and reproducibility. Continued innovation in bioinks, optimization of bioprinting parameters, and improved strategies for tissue maturation and integration are essential for the translation of these constructs from the laboratory to clinical settings. (3) Lastly, Chapter 3 aims to develop a biomimetic 3D scaffold tailored for the delivery of epithelial cells. The commercial bioink Lifeink® 220 (Cellink, Bico Company) was loaded with keratinocytes to print linear tubes into different scaffold geometries utilizing a BioAssemblyBot 200 bioprinter from Advanced Solutions. For the bioink, bioprinting parameters were set and printability and stability were assessed. Cellular responses were analyzed based on cellular migration, number of cells, and cellular expansion units using fluorescent microscopy and customized tools for image analysis on QuPath software. Both the scaffold design and the initial cellular density were able to modulate cellular response and enhance area covering. Equations to translate these experimental outcomes to clinical settings were proposed. Overall, the techniques applied during this project show potentialsto enhance cellular response aiming wound healing. The results also demonstrate that the continuous development of 3D bioprinting systems is promising to improve clinical outcomes in open wounds.

COMMITTEE MEMBERS:
Externo à Instituição - ROGERIO MORAES CASTILHO - UMICH
Presidente - 1278451 - ELIETE NEVES DA SILVA GUERRA
Interna - 2528668 - PAULA ELAINE DINIZ DOS REIS
Externo à Instituição - RICARDO DELLA COLETTA - UNICAMP
Interna - 3342495 - TAIA MARIA BERTO REZENDE