Banca de DEFESA: Joyce Silva dos Santos

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Joyce Silva dos Santos
DATE: 26/08/2024
TIME: 14:00
LOCAL: Plataforma Virtual Google Meet - ACESSO https://meet.google.com/ezh-ybbd-mpu
TITLE:

Evaluation of the quality and biological activity of topical formulations based on rosehip oil (Rosa aff rubiginosa)

KEY WORDS:

emulsions, vegetable oils, rosehip, medicinal plants, pharmaceutical technology.

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Introduction: The skin is an important organ of the human body that, serving as an interface between the internal and external environment for protection, is constantly exposed to various stress factors. Natural products stand out for their properties that are compatible with the dermal mantle and their composition, which is both therapeutic and protective for the skin. Rose Hip Oil (Rosa aff rubiginosa) has traditionally been used to combat skin aging, as a skin lightening agent, a scar reducer, and a healing agent. Although it is easily found in pharmacies in Brazil, few studies have been conducted to prove the efficacy of this oil in skin and wound care. Objective: To evaluate the quality and safety of topical formulations (emulsions and microemulsions) developed with rose hip oil for use in clinical practice. Methods: Two emulsions containing 30% rose hip oil were prepared using the phase inversion method, differing only in the presence or absence of the synthetic antioxidant BHT (2,6-Ditert-butyl-p-cresol). The emulsions, along with a sample of pure oil, were placed in airless containers and kept in a climate chamber at 40°C and 35% relative humidity, undergoing accelerated stability tests for 90 days, during which organoleptic, microbiological, physical, and chemical parameters were evaluated. Pseudoternary phase diagrams for the preparation of rose hip oil microemulsions were also developed, employing a low-energy method, using Tween 80 as a surfactant and testing two different cosurfactants: glycerin and propylene glycol. The best microemulsions were stored for seven days in an oven at 40°C and 35% relative humidity and were evaluated for pH, conductimetry, granulometry, and microbiological study. Both emulsions and microemulsions were subjected to safety tests (irritability, cell viability, inflammatory profile), in vitro skin permeation tests on Franz cell models, and ex vivo healing potential. Permeation analyses were performed using HPLC, using linoleic acid as the analyte of interest.Results: It was possible to develop an emulsion with 30% rose hip oil, whose organoleptic characteristics remained stable for 90 days when stored in the climate chamber. The oil incorporated into the emulsions prepared with the synthetic antioxidant maintained its antioxidant activity over the storage time, while the pure oil showed significant degradation of this parameter from the 15th day of storage. The emulsion prepared without synthetic antioxidant lost significant antioxidant activity, comparable to pure oil. Regarding the development of the microemulsion, the use of glycerin was more efficient compared to propylene glycol, allowing the incorporation of up to 5% oil. The pure oil, the emulsion, and the microemulsion showed no signs of irritation in the HET-CAM (Hen’s egg test chorionallantoic membrane), cellular toxicity, or inflammatory action. In the permeation study, none of the tested formulations showed absorption, indicating a local action. The emulsion containing 30% rose hip oil was more effective in promoting the penetration of linoleic acid into the dermis compared to pure oil and the microemulsion. Although without significant differences, the ex vivo healing study showed a greater collagen density in the group treated with the rose hip microemulsion, along with a significant increase in TGFβ release.Conclusion: The emulsion containing 30% rose hip oil and the microemulsions containing 1.25% to 2.5% showed good stability and microbiological quality, and were considered safe for cutaneous use. The emulsion was more effective in stimulating the penetration of linoleic acid into the deeper layers of the skin. Although it did not facilitate the penetration of linoleic acid, the rose hip microemulsion increased TGFβ release and collagen density in lesions created on human skin.

COMMITTEE MEMBERS:
Externa ao Programa - 1697332 - DANIELA CASTILHO ORSI - nullExterno à Instituição - IDEJAN PADILHA GROSS - UnB
Externa ao Programa - 2495888 - IZABEL CRISTINA RODRIGUES DA SILVA - nullPresidente - 1750177 - LIVIA CRISTINA LIRA DE SA BARRETO
Externa à Instituição - TALITA FARAJ FARIA - SARAH