Banca de DEFESA: RAQUEL FIGUEREDO RAMOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAQUEL FIGUEREDO RAMOS
DATE: 13/09/2024
TIME: 14:30
LOCAL: Modalidade híbrida - SALA INTERNA DA DIREÇÃO FS - PLATAFORMA VIRTUAL MICROSOFT TEAMS
TITLE:

Endodontic sealers and periapical response: antimicrobial effects, toxicity, healing and immune response

KEY WORDS:

Antimicrobials, bioceramics, biocompatibility, toxicity, bioactivity

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

The introduction of bioceramic-based materials in endodontics was of great importance. Bioceramic sealers help to bury microorganisms by sealing the dentinal tubules and isolate the communication between the root canal and the periradicular space. Furthermore, these materials demonstrate bioactivity in periradicular tissues. Thus, this study evaluated the potential of extracts of the bioceramic sealers bioc sealer and bio-c sealer ion + in comparison with the ah plus sealer, in primary culture of human periodontal ligament cells (hpdlscs). Initially, the antimicrobial activity of the sealer extracts was verified by evaluating the minimum inhibitory concentration for the growth of enterococcus faecalis and candida albicans. Subsequently, the toxicity of the sealer extracts was verified in culture of hpdlscs by the mtt assay. Next, the proliferative and migratory effects of these sealers were evaluated by the scratch assay in hpdlsc cultures. Finally, the effect of the sealers on gene expression by pcr was evaluated, as were the pro-inflammatory cytokines tumor necrosis factor alpha (tnf-α), interleukin IL-1β and IL-6, and the regulatory cytokine IL-10, in different experimental situations. Results demonstrated that ah plus and bioc sealer ion + inhibited 100% of the growth of e. Faecalis in the presence ofextracts (1:1, 1:2 and 1:4). However, only the 1:1 extracts of ah plus, bio-c sealer and bio-c sealer ion + inhibited 100% of the growth of c. Albicans. The toxicity of the cements was demonstrated in a dose-dependent manner in hpdlsc cultures, so that the lowest viability percentages were observed in the presence of the extracts of ah plus (1:1; 55%), bio-c sealer (1:1; 69%) and bio-c sealer ion + (1:1; 72%). In the scratch assay, it was observed that the extract of the bio-c sealer ion + cement (1:4) allowed the highest rate of cell migration and proliferation after 48h, comparedto the data of the ah plus and bio-c sealer cements. Finally, it was observed that ah plus up-regulated the gene expression of the cytokines tnf-α, IL-6 and IL-10 in all conditions tested in vitro. Conversely, the bio-csealer and bio-c sealer ion + cements did not affect the gene expression of tnf-α, IL-6 and IL-10 in all conditions. Ah plus also increased IL-1β gene expression compared to bio-c sealer under lps and ifn-γ-stimulated conditions. Thus, it is concluded that bioceramic sealers showed satisfactory antimicrobial activity in the most concentrated extractsagainst e. Faecalis and c. Albicans, with moderate toxicity. In addition, bioceramic sealers allowed wound closure in vitro and maintained inflammatory and anti-inflammatory cytokines at basal levels. Given these results, accidental periapical extravasation of bioceramic-basedsealers does not appear to have negative consequences for periradicular repair”.

COMMITTEE MEMBERS:
Interna - 1278451 - ELIETE NEVES DA SILVA GUERRA
Externa à Instituição - FRANCINE BENETTI - UFMG
Externo ao Programa - 1752418 - JACY RIBEIRO DE CARVALHO JUNIOR - nullPresidente - 3342495 - TAIA MARIA BERTO REZENDE