Banca de DEFESA: MARIA ESTER FRANÇA DE MELO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA ESTER FRANÇA DE MELO
DATE: 09/02/2026
TIME: 14:00
LOCAL: Sala interna da direção
TITLE:

Integrated Analysis of the Antimicrobial Activity, Elemental Composition, and Biological Effects of the Biomaterials Bio-C Temp and UltraCal XS on Human Peripheral Blood Mononuclear Cells and Periodontal Ligament Cells In Vitro

KEY WORDS:

Intracanal medication, bioceramics, cell viability, cell migration, inflammation

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Endodontic treatment aims to disinfect the root canal system and properly prepare it for obturation, with calcium hydroxide being the most widely used intracanal medication. However, new bioceramic materials have been proposed to enhance antimicrobial activity and biological response in periapical tissues. This study aimed to integrative evaluate the elemental composition, antimicrobial activity, and biological effects of Bio-C Temp and UltraCal XS on peripheral blood mononuclear cells (PBMCs) and human periodontal ligament cells (hPDLSCs). Elemental composition was determined by X-ray fluorescence, while calcium ion release and pH variation were monitored at different experimental periods. Antimicrobial activity was assessed against Enterococcus faecalis, Staphylococcus aureus, and Candida albicans by determining the minimum inhibitory concentration and the minimum bactericidal and fungicidal concentrations. Biological evaluation included cell viability assays using the MTT method, cell proliferation assessed by trypan blue exclusion assay, cell migration by the scratch assay, nitric oxide production quantified by the Griess method under inflammatory stimulation with lipopolysaccharide (LPS) and LPS associated with interferon gamma (IFN-γ), morphological analysis by scanning electron microscopy, and gene expression analysis of inflammatory cytokines by real-time polymerase chain reaction. Both materials showed calcium as the main component, with a predominance of barium in UltraCal XS and tungsten in Bio-C Temp, in addition to secondary radiopacifiers. UltraCal XS exhibited higher initial calcium ion release and greater alkalinity, whereas Bio-C Temp showed more stable ion release over time. Both materials inhibited the growth of E. faecalis and showed partial inhibitory effects against S. aureus, with greater efficacy observed for UltraCal XS, while only this material exhibited antifungal and fungicidal activity against C. albicans. UltraCal XS maintained PBMC viability above 97% at all dilutions, whereas Bio-C Temp showed significant cytotoxicity at higher concentrations, with progressive recovery at higher dilutions. In hPDLSCs assays, both materials reduced cell viability at higher concentrations, with recovery at higher dilutions. UltraCal XS stimulated cell migration and proliferation, whereas Bio-C Temp significantly inhibited these parameters at higher concentrations. Both materials modulated the expression of TNF-α, IL-1β, IL-6, and IL-10 in a stimulus-dependent manner. It is concluded that UltraCal XS presents a more favorable biological profile, whereas Bio-C Temp, despite its adequate physicochemical properties, exhibits greater cytotoxicity and negative effects on cell migration and proliferation at higher concentrations.

COMMITTEE MEMBERS:
Presidente - 3342495 - TAIA MARIA BERTO REZENDE
Externa ao Programa - 3437499 - ANA LIVIA GOMES CORNELIO - UnBExterno ao Programa - 1752418 - JACY RIBEIRO DE CARVALHO JUNIOR - UnBExterno à Instituição - Marcos Coelho Santiago - IOA