Banca de DEFESA: Natane Castelo Branco Duarte

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Natane Castelo Branco Duarte
DATE: 24/08/2022
TIME: 13:00
LOCAL: Plataforma Teams
TITLE:

Development Of Innovative Effervescent Sanitization Device Containing Effective Multiparticle System Agaisnt Sars-Cov2 And Other Infectious Agents

KEY WORDS:

pandemic, covid-19, sars-cov 2, coronavirus, efervescent tablet, effervescent device, thymol, chlorhexidine, microemulsion, sanitizer

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The emergence of the COVID-19 pandemic, as well as recent outbreaks caused by old contagious pathogens, have raised the alarm about the rise of unknown or unexplored infectious diseases. Studies show that areas such as toilets can be important means of disease transmission. The main potential routes are: the fecal-oral route, the direct contact with surfaces or fomites, and the respiratory route, through exposure to droplets and bioaerosols generated by flushing toilets. In this respect, it is essential that new technologies that are effective in preventing the spread of disease be created. The objective of this work was to develop an innovative effervescent formula which contains recycled soap, and low toxicity active agents with broad spectrum of action, thymol, and chlorhexidine digluconate, for the sanitization of water, air, and environments. In addition, it can help the cleaning and disinfection of surfaces of toilets, urinals, sinks, and pipes, and it can be used as a protection agent. Initially, an analytical methodology was developed in high performance liquid chromatography to quantify the two active agents simultaneously. The method presented adequate validation parameters and was selective against the formulation components. The granulated soap was developed from recycled oil and a sodium hydroxide aqueous solution. Different ratios were tested until a pH close to 8 was obtained. Microemulsions were formulated to incorporate the active agents, and they were characterized as to pH, hydrodynamic diameter, polydispersity index (PDI), zeta potential and content. The selected formulation presented a pH of 7.39, a diameter of 588.83 nm, a PDI of 0.202, and a zeta potential of 34.9 mV. In the accelerated stability study, the pH remained neutral, and the zeta potential was positive. The droplet diameter and the PDI suffered greater changes in the samples, which were stored at elevated temperature. There was a reduction in the chlorhexidine content, and the thymol content remained stable. The microemulsion was used to produce effervescent granules, which were mixed with soap to form a multiparticulate system, followed by molding to form tablets, which showed pharmacotechnical parameters within acceptable limits, and disintegration time in the range between 8 and 10 minutes. The bactericidal (Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923), fungicidal (Candida krusei ATCC 6258) and virucidal (Adenovirus HAdV-2 and Coronavirus Murino - IV (MHVIV) efficacy of the microemulsion and the effervescent tablet was tested. The concentration tested was 1.152 μg/mL of thymol, and 0.0021 mL/mL (0.21%) of chlorhexidine digluconate. The formulations completely inhibited the bacterial and fungal colonies that were present in the contaminated water. In viral models, there was 90% reduction for HAdV-2, and 99% for MHVIV, although there was apparent toxicity of the cell culture. The chemical compatibility between the active agents and other raw materials was evaluated by thermal analysis. The results showed no incompatibilities, in addition which indicates that a possible protection of surfactants over thymol occurs. Thus, the developed product proves to be effective in eliminating pathogens in aqueous media, and it is a promising mechanism to prevent the spread of diseases. Further tests should be conducted to evaluate its toxicity as well as the effectiveness of the instrument in sanitizing air and surfaces. 

BANKING MEMBERS:
Externa à Instituição - JANAINA VERSIANI DOS ANJOS - UFPE
Externo à Instituição - RICARDO FERREIRA NUNES - IESB
Externa ao Programa - 1697332 - DANIELA CASTILHO ORSI
Interno - 1880131 - GUILHERME MARTINS GELFUSO
Presidente - 1750177 - LIVIA CRISTINA LIRA DE SA BARRETO