Banca de DEFESA: MAYARA ALVES DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MAYARA ALVES DE OLIVEIRA
DATE: 31/03/2025
TIME: 08:30
LOCAL: Plataforma Teams
TITLE:

EVALUATION OF THE REGENERATIVE POTENTIAL IN VITRO OF DECELLULARIZED PULPS AS BIOLOGICAL SCAFFOLD

KEY WORDS:

Pulpar Regeneration; Decellularization; Extracellular Matrix; Decellularized Pulp

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

“Endodontic treatment in young permanent teeth with incomplete root development is challenging. In this context, regenerative therapies using scaffolds derived from decellularized extracellular matrix (dECM) have shown great potential in promoting tissue regeneration and restoring pulp tissue vitality. A recent study identified the persistence of key proteins related to the endodontic regenerative process, such as Periostin (POSTN) and Fibrillin (FBN1), in dECM through proteomics in the two protocols used in this study. Thus, the aim of this study was to characterize decellularized pulp tissues, analyze the morphology of the dental pulp after the application of decellularization protocols, and evaluate the interaction of dental pulp stem cells (DPSCs) with the resulting dECM products. For this purpose, cellular morphology in contact with these tissues and their respective forms—extracts and lyophilized dental pulp—was analyzed. The decellularization of pulp tissues was conducted based on two distinct protocols: the protocol described by Matoug-Ewerfelli et al. (2018) and that of Song et al. (2017). Migration, proliferation, and cell viability assays were then performed. Pulp tissue characterization was conducted through DAPI staining and DNA extraction. Morphological changes were analyzed by scanning electron microscopy (SEM). Cell viability and proliferation were assessed using the trypan blue exclusion test, while cell migration ability was determined by the scratch assay. The results obtained demonstrated that decellularized pulp tissue presents great potential for regenerative endodontic therapy, highlighting the affinity of cells in contact with the matrices and their products, which were analyzed by SEM. Additionally, cytotoxicity assays showed that matrix-derived products did not cause cytotoxicity when in contact with cells, promoting a significant increase in metabolic activity. In the proliferation and migration assays, a quantitative increase was observed in the presence of all matrix-derived products, evidencing the regenerative potential of decellularized pulp tissue. Thus, decellularized scaffolds derived from dental pulp show great potential for applications in regenerative endodontic treatments, promoting the repair of pulp lesions and root development. However, further studies are necessary to optimize decellularization protocols and evaluate the long-term clinical response of this therapy.”

COMMITTEE MEMBERS:
Presidente - 3342495 - TAIA MARIA BERTO REZENDE
Externa ao Programa - 3437499 - ANA LIVIA GOMES CORNELIO - UnBExterno ao Programa - 1752418 - JACY RIBEIRO DE CARVALHO JUNIOR - UnBExterna à Instituição - KELY FIRMINO BRUNO - FASAM