Banca de DEFESA: FRANCIELE SCHLEMMER

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : FRANCIELE SCHLEMMER
DATE: 17/06/2025
TIME: 14:00
LOCAL: A definir
TITLE:

MicroRNA signature and PARP1 expression as biomarkers of therapeutic response in triple-negative breast cancer. 

KEY WORDS:

“ Triple-negative breast cancer (TNBC), microRNAs, PARP1, Biomarkers, Pathological Complete Response (pCR), Paclitaxel, The Cancer Genome Atlas (TCGA) ”

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

“ Breast cancer is a complex and heterogeneous disease, with histologically similar tumors often displaying variable prognoses and therapeutic responses. Triple-negative breast cancer (TNBC) accounts for approximately 15% of all cases and is primarily treated with chemotherapy. The assessment of pathological complete response (pCR) following neoadjuvant chemotherapy is performed using the Residual Cancer Burden (RCB) index. Approximately one-third of TNBC patients achieve pCR, which is associated with a favorable long-term prognosis and a five-year disease-free survival rate exceeding 90%. In this context, mutations in genes involved in DNA repair pathways have been investigated as potential biomarkers to guide targeted therapies, such as poly (ADP-ribose) polymerase (PARP) inhibitors. Moreover, microRNAs have emerged as promising diagnostic and prognostic biomarkers due to their role in regulating gene expression via RNA interference, impacting both oncogenic and tumor suppressor pathways. This study investigated the expression profile of microRNAs and PARP1 in breast cancer cell lines and formalin-fixed paraffinembedded (FFPE) tumor samples from patients with TNBC, in addition to analyzing clinical and genomic data from The Cancer Genome Atlas (TCGA). The cell lines examined included MCF-7 (hormone receptor-positive, HR+), MDA-MB-231 (TNBC, BRCA1-proficient), and MDA-MB-436 (TNBC, BRCA1-mutated). Significant differences were observed in the expression levels of miR-7, miR-21, miR-671, and miR-146a among the cell lines, along with changes in response to paclitaxel treatment and PARP1 expression levels, which were correlated with the microRNA profiles. In FFPE tumor biopsies, miR-7, miR-21, and miR-146a were identified as potential predictors of pCR in TNBC patients. ”

COMMITTEE MEMBERS:
Externa à Instituição - DANIELE ASSAD SUZUKI - SÍRIO
Interno - 1764941 - DIEGO MADUREIRA DE OLIVEIRA
Externo ao Programa - 1643347 - FABIO PITTELLA SILVA - nullExterna à Instituição - PAULA FONTES ASPRINO - SÍRIO
Presidente - 1127261 - RICARDO TITZE DE ALMEIDA