Banca de DEFESA: Artur Fiuza Borges Arantes

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Artur Fiuza Borges Arantes
DATE: 04/12/2025
TIME: 14:00
LOCAL: Auditório 2
TITLE:

“Development of topical formulation for skin depigmentation containing hydroalcoholic extract of Morus nigra L”

KEY WORDS:

“Morus nigra; tyrosinase; melanin; hyperpigmentation.”

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

“Skin hyperpigmentation can be characterized as a disorder in which there is excessive melanin deposition. The causes of hyperpigmentation are diverse and can range from patient stress to metabolic disorders and responses to skin trauma. During the stages of the melanogenesis process, tyrosinase acts as the main enzyme in the cascade of melanin production reactions, therefore, it can be considered an excellent target of pharmacological interest for the development of topical formulations. The production of medicines and cosmetics from medicinal plants and their extracts is a historical and current reality, with trees of the genus Morus being part of this context. Previously described studies in the literature demonstrated the inhibitory activity of the crude ethanolic extract of their leaves against the enzyme tyrosinase; however, to date, there are no studies regarding the potential of the extract after chlorophyll removal and incorporation into formulations. Therefore, this project aimed to produce, characterize, and formulate a cosmetic from the hydroalcoholic extract obtained from the leaves of Morus nigra L. The collected plant material was oven-dried at 40°C for 5 days, pulverized, and characterized by weight/particle size and moisture content. It was then subjected to ethanolic extraction by cold maceration (1:5 m/v), filtered, and rotary evaporated. To remove chlorophyll, the extract was resuspended in a methanol/water hydroalcoholic solution (1:1 v/v), followed by centrifugation at 4°C at 10,000 rpm for 10 min. The supernatant was collected, rotary evaporated again, and lyophilized. A total of six batches of extract were produced. The extracts were characterized by total solids content, extractive yield, thin-layer chromatography, and high-performance liquid chromatography associated with a diode array detector. The extract's inhibition potential against the enzyme tyrosinase and its cytotoxic effect on three cell lines: mouse fibroblast L929, murine melanoma B16F10, and human keratinocyte HaCat were evaluated. Two formulations containing the extract were also produced: a nanoemulsion and a serum. Both formulations were characterized by pH, viscosity, particle size, polydispersity index, zeta potential, and conductivity. They are currently being evaluated in an accelerated stability assay. Tyrosinase inhibition was above 90% for all extract batches at concentrations above 20 µg/mL; the overall average IC50 for the crude batches was 9.79 µg/mL and 10.81 µg/mL for the clean batches. The IC50 values for the extract's cytotoxicity on the three cell lines tested were all above 100 µg/mL. After six months of preparation, the formulations maintained their properties with little change and continue to be monitored in stability tests.”

COMMITTEE MEMBERS:
Externa à Instituição - MARCELA MEDEIROS DE FREITAS - MS
Externo à Instituição - EDEMILSON CARDOSO DA CONCEICAO - UFG
Interno - 1880131 - GUILHERME MARTINS GELFUSO
Presidente - 1562111 - PEROLA DE OLIVEIRA MAGALHAES DIAS BATISTA
Interna - 1844214 - YRIS MARIA FONSECA BAZZO