Banca de DEFESA: MIRIAM MONTEIRO ALVARES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MIRIAM MONTEIRO ALVARES
DATE: 16/09/2025
TIME: 14:30
LOCAL: A definir, em função da banca.
TITLE:

TRIPLE-NEGATIVE BREAST CANCER IN A POPULATION OF THE FEDERAL DISTRICT – ANALYSIS OF TUMOR-INFILTRATING LYMPHOCYTES AND THEIR CORRELATIONS WITH CLINICAL PHENOTYPES AND TISSUE EXPRESSION OF THE BIOMARKER MICRORNA miR-155

KEY WORDS:

Triple-negative breast cancer; TILs; biomarkers; microRNA; miR155.

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Various environmental and genetic risk factors are associated with the development of breast cancer. Diagnosis is based on clinical, imaging, and histopathological factors. Among the molecular variations of breast cancer, when there is no expression of hormone receptors and HER2, the tumor is classified as triple-negative (TNBC). Neoadjuvant chemotherapy is the preferred approach for patients with these locally advanced tumors, and the presence of overexpressed tumor-infiltrating lymphocytes (TILs) is linked to favorable treatment outcomes, serving as prognostic markers. Additionally, microRNAs can function as biological markers. Studies have shown that miR-155 levels are elevated in cases of ductal carcinoma of the breast. Understanding miR-155 as an oncogenic marker in breast cancer is crucial for associating it with potential therapeutic targets. This study employed a cross-sectional design, utilizing biopsy and surgical samples from patients with TNBC after neoadjuvant chemotherapy in the Federal District. Analyses were conducted to identify associations between TILs expression and treatment response, focusing on tumor markers such as CD3 and miR-155. Clinical data were collected, and molecular analyses were performed using PCR, while marker expression was assessed through immunohistochemistry. Logistic regression tests and ROC curves were applied to evaluate the proposed associations, with a significance level set at p < 0.05. The project received proper approval and had the necessary infrastructure for execution. The study found that 44.1% of patients who underwent neoadjuvant chemotherapy achieved a complete pathological response (pCR). The analysis of lymphocytic markers revealed that CD3 expression was statistically significant as a predictor of TILs expression, particularly when combined with microRNA miR-155. The combination of miR155 and CD3 achieved an accuracy of 98% in predicting TILs expression, with 100% sensitivity and 87.5% specificity. These findings suggest that incorporating miR-155 as an additional marker may improve predictions regarding TILs expression in TNBC and consequently enhance treatment response.

COMMITTEE MEMBERS:
Interno - 1764941 - DIEGO MADUREIRA DE OLIVEIRA
Externo à Instituição - FABRICIO TAVARES MENDONÇA - HBDF
Externa à Instituição - MARIA DE FATIMA BRITO VOGT - OUTROS
Externo à Instituição - RAPHAEL SEVERINO BONADIO - UnB
Presidente - 1127261 - RICARDO TITZE DE ALMEIDA